Poster Presentation Asia-Pacific Vaccine and Immunotherapy Congress 2023

Glycolipidated-liposomal peptide-based vaccine induces protection against Streptococcus pyogenes upper respiratory tract infection. (#174)

Victoria Ozberk 1 , Mehfuz Zaman 1 , Ailin Lepletier 1 , Sharareh Eskandari 1 , Jacqualine Kaden 1 , Jamie-lee Mills 1 , Ainslie Calcutt 1 , Emma Langshaw 1 , Jessica L Dooley 1 , Yongbao Huo 1 , Glen Ulett 2 , Michael Batzloff 1 , Michael Good 1 , Manisha Pandey 1
  1. Institute for Glycomics, Upper Coomera, QLD, Australia
  2. Menzies Health Institute Queensland, Griffith University, Gold Coast , QLD, Australia

Mucosally active subunit vaccines are an unmet clinical need due to lack of licensed immunostimulants suitable for vaccine antigens.  We show that intranasal administration of liposomes incorporating: the Streptococcus pyogenes peptide antigen, J8; diphtheria toxoid as a source of T-cell help; and the immunostimulatory glycolipid, 3D(6-acyl) PHAD® (PHAD), was able to induce both cellular and humoral immunity.  We demonstrate long-lived humoral and cellular memory responses following vaccination. However, mice genetically deficient in either mucosal antibodies or total antibodies were protected against S. pyogenes respiratory tract infection.  By using IL-17-deficient mice or by depleting cellular subsets using antibody therapy, we show that the cellular responses encompassing, CD4+ T-cells, IL-17, macrophages and neutrophils play critical roles in vaccine-mediated mucosal immunity. Overall, the data demonstrate the utility of a novel mucosal vaccine platform to deliver multi-pronged protective responses against a highly virulent pathogen.